Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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Index of similarity among genotypes were calculated by using NTsys ver. Email this article Login required. Article Tools Print this article. First group consisted of somatic hybrids nos. Based on the distribution of phenols and lignin content. Ihe second group 9 clones contained high lignin.

Seedless citrus improvement can be done by manipulating the level ploidy. JavaScript is disabled for your browser. This orange tastes sweet but relatively many seeds fruit seed.

In the fusion of Girilaya x Sidikalang. Sidikalang and TT 75 were tested for their genetic variability and relationship. User Username Password Remember me. Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus.

The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore.

Result showed that Java patchouli Girilaya contained phenols and lignin respectively View original from ejurnal. Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs.


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DNA of the orotoplas materials used were extracted by using the modified method of orozcocastulo et al. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli Sidikalang and TT 75and the somatic hybrids. Industrial Crops Research Journal.

Language Select Language English Indonesian. One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus.

Abstract Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Metadata Show full item record.

Prosiding KPSDA

Haploid cell population repeatedly sub cultured every 4 weeks in a double layer media: Email the author Login required. The plant material used for the production of diploid callus is a population of diploid cells induced from embryonic nucellar tangerine. Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Somatic hybridization by fusion technique is a breakthrough technique that allows the plant generated by an increased level ploidy. The strategy chosen to assemble the triploid tangerine because the technique of protoplast isolation, fusion and regeneration of tangerine with diploid level has been mastered ploidy.

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Fusi protoplas interspesifik aspergillus Spp

Pre cold treatment protoplzs more than 3 days on the response forming callus from pretreatment less than 3 days. S Assessment of genetic variability of patchoulli Pogostemon sp. Pusat Penelitian dan Pengembangan Perkebunan Jln.


Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs. How to cite item. Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan.

The best enzyme formulations for the isolation of protoplasts from diploid callus and leaves in vitro and protoplast Haploid pollen is macerozim 0. Five random primers, OPD The second group was then classified into 2 minor groups. OPH 19 and Abi Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian Ihe somatic hybrids can be categorized in three group The irst groups 5 clones contained high phenols higher than the average.

Plants with triploid ploidy level usually have sterile fruit seedless. Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al the laboratory of the Research Institute for food Crop Biotechnology, Bogor in November Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.